The Chilean Matorral has a high degree of plant endemism and a high vulnerability due to anthropogenic pressures, mainly anthropogenic fires, which is why it is currently recognized as a biodiversity hotspot. There is evidence that fire can reduce the richness and abundance of microorganisms in the soil, including mycorrhizal fungi, thus modifying their diversity. On the other hand, there is evidence that after a fire it is possible to find trees that survive and are able to resprout, so they function as nurse plants and can in turn be a source of mycorrhizal fungi for a potential recovery of the affected system. That said, in this study we aim to test the effect of fire and nurse plants on the diversity of arbuscular mycorrhizal fungi in the sclerophyllous forest. This forest is the most representative vegetation formation of the Chilean Matorral and the dominant plant species present there form arbuscular mycorrhiza. A lower diversity of arbuscular mycorrhizal fungi is expected in sites affected by fires and without the presence of nurse plants compared to sites not affected by fires and with the presence of resprouting nurse plants. This project includes work with local communities in order to transfer knowledge among all participants.

The study site is located in a split structure within the fluvial wetland of the Rio Uruguay, the largest of its kind in Uruguay. The site offers a natural experiment for studying vegetation succession, with three areas at clearly defined stages: mature, intermediate, and neoformation, progressing from north to south. Although the ecosystem holds significant conservation value, it faces serious threats from the invasive legume Gleditsia triacanthos, which is displacing native species in parts of the riparian forest. In Uruguay, Gleditsia triacanthos is recognized as one of the two most invasive woody species, posing a significant threat to native forests due to its ability to outcompete and replace native vegetation. Soil microbial communities play key roles in plant health and establishment, thus, we aim to investigate how this invasive species interacts with the soil microbial community and how this interaction differs from that of native species. This site presents an opportunity to enhance our understanding of the relationship between a non-native invasive species and the native microbial community within the framework of ecological succession.

Kakamega forest is the only forest of its kind, the remnant of the Guineo-Congolian equatorial rainforests occurring in Kenya. It is an Important Bird Area (IBA), rich in tree species and a home to endemic plants and animals. The forest is in IUCN, 2015 priority list for conservation of indigenous forest. Its global significance earned Kakamega the UNESCO World Heritage Site title in 2010.  However, despite this significant global recognition, the biodiversity in this area is under threat due to human activities.  Over 100 years the forest has undergone major land use changes such as conversion of indigenous forest to plantations, forest fragmentations, degradation and various human activities. Consequently, over 50% forest cover has been lost in the last 38 years and habitats suitable for the survival of biodiversity are greatly threatened. Currently seven habitats types are evident. They include the indigenous primary and secondary forests, secondary plantation forest (exotic and indigenous tree species), open grasslands, riverine and swamp sites and the adjacent mono and mixed crop farmlands.  

This study seeks to understand how land use changes have affected the occurrence and the diversity of soil mycorrhizal fungi in the study area. The project will use the recommended SPUN protocols for soil samples collection and used molecular techniques to quantify the mycorrhiza fungi.  The project will involve the community and other stake holders in samples collection, processing and training on mycorrhiza. Results from the project will be disseminated through workshops, local and international conferences, and publications. It is envisaged that the community will be sensitized on the soil mycorrhizal fungi, their role to plants, threats and the need to conserve the habitats leading to their protection and conservation actions.

This project will explore how mycorrhizal fungi and microbial communities contribute to soil health and carbon/nutrient dynamics across India’s diverse ecosystems. Through soil sampling in the Himalayan forests of Mukhteshwar, the tropical zones of Guwahati, coastal Goa, and urban Delhi, the study will aim to uncover patterns of fungal and microbial diversity shaped by both ecological richness and human impact. Using whole metagenome sequencing (WMS), we will generate high-resolution profiles of soil microbiomes, focusing on taxonomic diversity, functional genes, and their roles in carbon and nutrient cycling. The findings will enhance our understanding of interactions between mycorrhiza and other soil microbes across eco-regions, and will provide evidence-based recommendations for regenerative land management and improved soil health. This project will contribute to SPUN’s global mapping efforts and highlight the ecological intelligence beneath our feet.

The Palouse prairie of Washington (USA) is an endangered habitat, where less than 1% of the habitat remains due to land conversion. Restoration of the Palouse prairie may require reintroduction of plant and fungal species, if native fungi have been disrupted by non-native plants. Some late-successional native plants of conservation interest have high reliance on native arbuscular mycorrhizal (AM) fungi. However, the establishment of fungi and plants may depend on whether roots are pre-colonized with fungi prior to transplanting into field plots or inoculated at the time of planting. In this study, we will evaluate the impact of pre-colonization with soil fungi from a remnant prairie on fungal and plant establishment. The native plant, Silene spaldingii (Threatened), was grown in a greenhouse with or without soil from a remnant prairie. Uninoculated plants were inoculated during transplanting into field plots. I will compare 1) the community structure of soil fungi in the remnant prairie and the restoration area prior to, and one year after, restoration (SSU, ITS2), 2) the growth of S. spaldingii and 3) development of seeded plant communities. This work will provide insights into how pre-colonization with native fungi influences the establishment of fungi and plants in restored grasslands.

Intensive gold mining activities in Central Sulawesi have caused severe soil degradation and ecosystem disruption within the biodiversity-rich Wallacea region. The extensive mining operations have compromised soil integrity and natural ecosystem recovery processes, creating an urgent need for effective restoration strategies. This study investigates the potential of indigenous mycorrhizal fungi as agents for ecological restoration in degraded mining soils. Mycorrhizae establish beneficial symbiotic relationships with plants, enhancing nutrient uptake, improving stress tolerance, and restoring soil structure. By identifying and characterizing stress-tolerant mycorrhizal fungi native to the region, this research aims to develop biologically-based restoration approaches that leverage below-ground biodiversity to accelerate ecosystem recovery and support sustainable land management practices in post-mining landscapes. The findings will contribute to understanding how indigenous soil microorganisms can be utilized for effective rehabilitation of degraded mining areas, ultimately supporting both biodiversity conservation and sustainable land use in one of the world's most biodiverse regions. This research addresses the critical need for scientifically-grounded restoration methods that can restore ecosystem function while preserving the unique endemic biodiversity characteristic of the Wallacea biogeographical region.

Arbuscular Mycorrhizal Fungal Communities in Dalbergia Sissoo: Comparing Diversity in Wilted and Healthy Groves of Nepal’s Natural and Plantation Forests Ram Bahadur Khadka, Ph.D. Nepal Agricultural Research Council Abstract Dalbergia sissoo (Sissoo) is an ecologically and economically important tree species in Nepal, severely affected by Sissoo wilt disease. Arbuscular mycorrhizal fungi (AMF) play a crucial role in plant health and stress resistance, yet their association with D. sissoo in wilt-affected and healthy stands remains poorly understood. This study aims to compare AMF diversity and community composition in the rhizospheres of healthy and wilted D. sissoo trees across natural and plantation forests in Nepal. Soil and root samples will be collected from selected groves, and AMF spores will be isolated and identified using morphological and molecular techniques. Differences in AMF richness, diversity, and abundance between healthy and diseased trees, as well as between natural and plantation forests, will be analysed. The findings will provide insights into the role of AMF in Sissoo wilt resilience and contribute to sustainable forest management strategies for mitigating the sissoo decline. Keywords: Dalbergia sissoo, arbuscular mycorrhizal fungi (AMF), Sissoo wilt, fungal diversity, Nepal, forest health.

Mycorrhizal fungi form mutualistic associations with plant roots, they provide mineral nutrients to the plant and in return obtain carbohydrates. Mycorrhizal fungi are also indicators of the health of forest ecosystems and they are key in promoting the growth of plants thereby increasing plant diversity. Many mycorrhizal fungal communities across the globe remain underexplored including in countries like Uganda. In this project, we aim to determine the diversity of Mycorrhizal fungi in one of Uganda’s forest ecosystems of Bwindi Impenetrable National Park. The forest is regarded as one of the most significant forest ecosystems in Uganda, known for the conservation of huge biodiversity and home to almost half of the world's total mountain gorillas. It is an Afromontane forest with various distinct ecoregions/zones characterized by high-altitude montane bamboo, low-altitude mixed mature forest, and medium-altitude tropical evergreen forest which are expected to support a high diversity of fungal communities. We hypothesize that these different microclimates of Bwindi Impenetrable National Park could affect the diversity of mycorrhizal fungi communities. The project is expected to accomplish the following objectives: 1) Determining the diversity of mycorrhizal fungi in Bwindi Impenetrable National based on metabarcoding using soil and root DNA 2) Mapping the distribution of mycorrhizal fungal species in the three altitudinal zones of Bwindi Impenetrable National Park and 3) Determining the physical-chemical parameters of rhizosphere soil collected from the climatic regions of Bwindi Impenetrable National Park. A total of 36 root and soil samples will be collected from three altitudinal zones of the forest using SPUN’s standardized protocols. The Mycorrhizal fungal communities will be assessed using ITS2 rDNA metabarcoding. Studying mycorrhizal fungal communities in Bwindi Impenetrable National Park will give us a thorough understanding of the Bwindi’s forest ecosystem function. Furthermore, mycorrhizal fungal sequences from Bwindi Impenetrable National Park will provide first-time insights into the diversity of forest mycorrhizal fungal communities of Uganda.

Rising temperatures in tundra drive heightened wildfire activity, profoundly affecting fungal communities. Recent research highlights the significant influence fungi have on ecosystem carbon balance in northern ecosystems. Fungi impact primary production through plant nutrient acquisition, soil organic matter formation, and C losses via priming. Understanding post-fire shifts in fungal diversity is crucial for predicting wildfire consequences and climate feedbacks. The Yukon-Kuskokwim Delta in Alaska remains relatively understudied. By analyzing fungal diversity across unburned, recently burned, and historically burned sites, we can assess the effects of fire on fungal communities. Initial findings suggest that shrub abundance increases in the first decade following fire, potentially favoring ectomycorrhizal and ericoid mycorrhizal fungi as host plants respond positively to low-intensity burning with strong impacts on soil carbon accumulation and cycling rates. Building on an ongoing investigation of wildfire impacts on components of ecosystem carbon balance, including fluxes of greenhouse gasses and carbon stored in plant biomass and soils, this study will reveal fungal taxonomic identities that may be important regulators of these ecosystem dynamics. Our study will provide new insights into the connections between fungal identities and biogeochemical processing in a critical yet understudied tundra region and support training opportunities for undergraduate students.

The project will be carried out in the main biodiverse mountain system of Guatemala, Sierra de las Minas, a particular geological and natural system originated from the encounter of ancient land blocks and tectonic plates. It is still now the natural limit in America of Holartic genera as Abies and Acer but contains many others that are distributed in Central America as well as many Neotropical genera.  The scope of this project is to identify molecularly the mycorrhizal composition of its different forest according to the altitudinal gradient.  And after then, 1. To identify the dominant fungal genera and species, 2. To identify those that could be useful for inoculum in forestry and 3. To determine or confirm the taxonomic identity of many fruit bodies of ectomycorrhizal mushrooms, mainly basidiomycetes in the orders Russulales, Boletales and Cantharellales collected before and during the sampling. This is the first large molecular study that will provide information about fungal diversity in the country.

This is an interdisciplinary nationwide project with more than 30 scientists from UNAM (National Autonomous University of Mexico). It is part of the University Program of Interdisciplinary Soil Studies (PUEIS) Developed primarily at the Institute of Biology, Institute of Ecology, Faculty of Sciences, and Faculty of Higher Education of Iztacala.

The soil constitutes the ecological interface that harbors the greatest biodiversity in terrestrial ecosystems. Soil regulates global biogeochemical cycles, supports biological diversity, activity, and productivity, and provides the nutrients necessary for plant growth. A conserved soil is a biodiverse soil maintaining ecological functions such as microbial activity, carbon and nitrogen mineralization, and enzymatic activity. Despite the importance of soil this resource is not renewable, and its conservation is threatened by mismanagement and the lack of effective communication strategies about it. In Mexico, 45% of the territory has degraded soils, which means that they have lost the ability to maintain their ecological function, their biodiversity, and their productivity. Part of the problem is the lack of data and information on biodiversity and soil function in large geographic areas of Mexico to strengthen decision-making aimed at its sustainable management.

The Atlas of Mexico’s Soil Biodiversity have three guiding principles (Research, Teaching and Society Outreach), each with the following objective. Research – To generate frontier knowledge using public biodiversity databases, collaboration networks and the most modern DNA sequencing technologies to produce the greatest possible amount of knowledge in the shortest time and at the lowest cost. Teaching – To generate learning elements for high school, undergraduate and postgraduate levels to promote knowledge of life in Mexican soils and the need to protect this non-renewable resource. Outreach – To create an interface for data display and analysis to provide information to decision makers that promote friendly management practices with soil diversity, as well as programs for its conservation and monitoring.

This project investigates how European beech trees adapt to climate change, particularly drought, through their partnership with ectomycorrhizal fungi. We study beech forests across nine sites in Romania, spanning from lowland areas to high mountain regions, including some of the country’s oldest and most pristine forests. These fungi play a crucial role in helping trees absorb nutrients, especially under stress. By examining how ectomycorrhizal communities vary with climate and elevation, we aim to better understand their role in supporting forest health. Using a combination of DNA sequencing, soil analysis, and plant trait data, our research reveals how forests respond to environmental challenges and highlights the importance of protecting belowground biodiversity as part of broader conservation efforts.

The main goal of our project is to advance the knowledge on the systematics and diversity of ectomycorrhizal clavarioid mushrooms in underexplored regions of Mexico. Genus Clavulina is a well-known ectomycorrhizal fungus with high diversity in Mexico. However, other clavarioid genera, like Clavulinopisis and Ramariopsis, are poorly known in our country and therefore deserve more attention. We will sample basidiomata in regions of Sierra Norte de Puebla and Sierra Madre Occidental, which include temperate and cloud forests. For all specimens we will obtain DNA sequences of ITS and 28S ribosomal loci, as well as detailed macroscopic and microscopic descriptions, which will allow us to identify new records and new species. Considering the high diversity of plant hosts and ecosystems, we hypothesize that the sampled region harbors a high diversity of undescribed species. On the other hand, the new information on Clavulina will help us to evaluate the conservation status of some known species using IUCN standards. Local people will be involved in the project by exchanging knowledge through workshops, increasing awareness of the local fungal diversity and the importance of its protection at all levels.

The Mono Biosphere Reserve of Benin represents an important component of the Benin wetland complex. Among this complex, mangroves represent an ecosystem rich in plant, animals, and fungi. In Benin, most of studies implemented in mangrove focused only on plants and animals without addressing fungi which plays a significant role in mangrove ecosystem functioning. However, mangroves are facing significant pressures and their area decreased from 5500 ha in 1995 to 1105 ha in 2015, representing a reduction of 80 % in 20 years. Thus, it becomes urgent to explore fungal diversity poorly documented until now. The objective of this project is to identify and quantify fungal taxa present in mangrove ecosystems and thus providing valuable information about their biodiversity and composition. This data is crucial for effective conservation and restoration efforts. It can help assess the impact of disturbances (human, water quality, etc.) on fungal diversity, identify indicator species, and guide the development of strategies to protect and restore mangrove ecosystems.

Nepal is a country with abrupt elevation gradient, ranges from 50 to 8848 meters above sea level, towards the North resulting in contrasting climate and habitat formation. This change in elevation and climate allows diverse habitat formation and floral and faunal diversity vary greatly across the regions. Furthermore, In the East-west gradient, the precipitation patterns greatly vary in the country. In the monsoon season (June-Aug), heavy precipitation occurs in the Eastern regions and the western region receives less rainfall. In the winter season, the Western region receives more rainfall than the Eastern region. However, the total rainfall in the winter season is lesser than monsoon season. This creates a different soil moisture regime in East-West regions. Therefore, elevation and climate gradient towards the North and precipitation gradient towards East-West make Nepal rich in floral and faunal (aboveground) biodiversity hotspots, however, soil (belowground) biodiversity hotspots are yet to be determined. We assume the change in elevation gradient and habitat types altered the soil biodiversity. Soil sampling across elevation, climate, and precipitation gradient will help us to elucidate the diverse soil flora and faunal diversity. We will also have an opportunity to understand and assess the soil biodiversity in higher elevation zones, which is unique to Nepalese landscapes. Currently, we are collecting soil samples (voluntarily) from each of the physiographic areas. We only have three paired sites for this assessment. We followed the Soil Biodiversity Network (SoilBON) protocols to collect, prepare, store, and ship the samples. These sites broadly cover the soil biodiversity status of Nepal but are limited to covering all habitat types towards North-South and East-West regions. Therefore, we would like to extend our sampling sites from 3-paired sites to additional 18-paired sites and the results will contribute directly to the sample pool of SoilBON.

The Indian Sundarbans, covering 4,260 square kilometers and are the world's largest contiguous mangrove forest. This ecoregion is UNESCO World Heritage and a Ramsar Site. Which are located in the Ganges-Brahmaputra-Meghna delta in the West Bengal Province of India-Bangladesh. The region is home to Heritiera fomes and Avicennia alongside famed Bengal tiger and other IUCN-listed animals dolphins and bird species. Santal, Munda, Oraon, Ho, and Bhumij are the indigenous groups that depend upon fishing, honey harvesting, and ecotourism. The Sundarbans serve a key role in carbon sequestration, controlling coastal hydrology, and functioning as natural storm barriers, thereby minimizing the impacts of climate change.The Indian Sundarbans' fungal diversity is threatened by rising sea levels, salinization, overtourism and climate change-driven habitat fragmentation, disrupting soil and water balances. Ineffective conservation policies prioritize flagship species over microbial biodiversity, leaving fungi vulnerable to habitat loss, undermining ecosystem services like carbon sequestration and coastal protection.

The project aims to investigate the belowground diversity of ectomycorrhizal fungi in the Himalayan subtropical broadleaf forests of Pakistan, a region characterized by diverse habitats and unique ecological niches. These forests, located in northern Pakistan, span altitudes ranging from 500 to 2,000 meters above sea level and are dominated by broadleaf tree species such as oak, maple, walnut, and rhododendron. Additionally, tall trees, including Sal, Terminalia, Bauhinia, Schima, and Castanopsis, contribute to the dense canopy, creating shaded understories ideal for fungal growth. Climbers and epiphytes further enhance biodiversity in these forests.

Our hypothesis proposes that environmental variables, including altitude, soil type, and tree species composition, exert significant influence on ectomycorrhizal fungal diversity within these forests. We anticipate that higher elevation sites will harbor fungal communities adapted to colder temperatures, while lower elevation areas may support different species due to warmer conditions. Soil properties such as pH, moisture levels, and nutrient availability are also expected to shape fungal community composition.

Molecular aspects of the project involve metabarcoding of the rDNA region, targeting both the ITS and LSU regions using fungal-specific primers. This approach will facilitate accurate identification and classification of fungal taxa present in soil samples, providing insights into ectomycorrhizal belowground diversity. Sampling procedures adhere to standardized protocols, ensuring careful collection and preservation of fungal specimens from designated sites within the Himalayan subtropical broadleaf forests. Morphological, anatomical, and molecular characterization of specimens will be conducted to provide a comprehensive understanding of ectomycorrhizal diversity.

The anticipated outcomes of the project include peer-reviewed publications, thesis chapters, and reports documenting fungal diversity and community structure in the Himalayan subtropical broadleaf forests of Pakistan. These findings will contribute to scientific knowledge, inform conservation policies, and guide future research directions in mycology and forest ecology. Furthermore, the evaluation of conservation status based on fungal diversity assessments will support evidence-based decision-making and conservation prioritization efforts.

Expedition engagement and outreach activities will involve scientific dissemination, community workshops, educational programs, and social media outreach. Through these initiatives, the project aims to foster collaboration between researchers and local communities, raise awareness about fungal diversity and forest conservation, and inspire action towards the preservation of Himalayan subtropical broadleaf forest ecosystems.

Overall, the project seeks to advance our understanding of ectomycorrhizal belowground diversity in the Himalayan subtropical broadleaf forests of Pakistan and contribute to the conservation and sustainable management of these critical forest ecosystems.

This project investigates the variety and adaptability of ectomycorrhizal fungi in Peru's nutrient-poor Amazonian forests, particularly in the Yungas forests of POTSOM POSHOLL and the white-sand forests of the Allpahuayo-Mishana Reserve. Our goal is to find fungal symbioses that promote ecosystem functioning through soil and root sampling, metabarcoding methods (ITS and AMF markers), and community involvement. In order to produce ecological knowledge that will direct soil conservation, restoration, and sustainable management in tropical forest landscapes. The project will evaluate how these fungi respond to human pressures.

This research seeks to redress the limited consideration that fungi have received in Nepal's research and conservation priorities, especially within the broader context of the Hindu-Kush Himalayan region. It aims to investigate mycorrhizal fungal diversity and composition in the Eastern Himalayan subalpine conifer forests of Sagarmatha National Park, known as the realm of Mount Everest (8848m). By examining three elevation levels (3000m, 3400m, and 3800m) and two land use types (natural forests and meadows), the study seeks to understand how mycorrhizal communities vary across different altitudes and land use patterns. To achieve this, the project will adhere to the SPUN sample collection guidelines and employ advanced molecular techniques, such as metabarcoding of the rDNA region. 

A significant aspect of this study is its emphasis on community involvement in the research process. The research findings will be shared through peer-reviewed scientific publications and outreach mediums. Moreover, the project aspires to contribute to educational materials, including to update brochures for Sagarmatha National Park and the national checklist of fungi in Nepal. Furthermore, by enhancing our understanding of mycorrhizal diversity, this research aims to highlight the vital role these fungi play in the Himalayan ecosystem and advocate for their preservation through conservation actions.

Ecological succession provides fundamental descriptions of ecosystem processes by studying vegetation composition and structure, and soil properties. However, information is scanty on soil microbial communities particularly fungi and their relationship to the process of succession. This proposal aims to examine the recuperation of soil fungal diversity during ecosystem development following shifting cultivation in sub-tropical forests of North-east India (part of both the Himalaya and Indo-Burma biodiversity hotspots) having steeply sloped terrain and high forest cover. On second location, the project proposal covers the increasing ages (up to 50 years old) since shifting cultivation in the eastern coast of India (i.e. central Odisha), and on third location, it will focus on an elevation gradient (spanning over 100 m to 2000 m tropical to temperate forest) in Mizoram (North-east Indian states) with undisturbed forests paired with secondary disturbed forests of 40 years of age using metagenome sequencing (as per SPUN’s standardized protocols). We will test the hypothesis that forest disturbance influences soil fungal community composition but that fungi re-converge on similarity to primary forests as secondary forests age (it takes about 10 years for bacteria). We further anticipate that it will take longer for community re-convergence in uplands than lowland due to the slower vegetation regrowth at higher elevations.

This project will provide clues to the linkages, mechanisms and feedback that regulate soil fungal community assembly during ecosystem development following shifting agriculture in highly biodiverse sub-tropical region and elevation and disturbance gradients (on tropical, sub-tropical and temperate forests) of North-east India, and Odissa (tropical deciduous forest) which are unexplored. The project output will be expanding our understanding on: a) how does forest disturbance alters the fungal communities? b) how fungal communities are shaped during ecosystem recovery? and c) how does forest altitude affects fungal community composition in Northeast Indian forests of Indo-Burma region? We anticipate at least two high-quality publications from this work as both region lacks molecular data on soil fungi and on shifting cultivation globally. Further, we anticipate that the project outcome may provide useful link to develop potential indigenous biofertilizers for sustaining shifting agriculture in the region. 

This project is Indian-led with collaborating team including Prof. S.K. Tripathi an ecosystem ecologist from Department of Forestry, Mizoram University along with his team, Dr. Ghosh an experienced microbiologist from Bose Institute, Kolkata and his team, and Dr Hombegowda working in the state of Odisha in the secondary forests. The team will be supported by Dr Francis Brearley broadly experienced in tropical plant-soil-fungal ecology who is already collaborating with Prof. Tripathi and published number of papers together.

Prof. Tripathi is already working with tribal communities and promoting organic farming practices in the region through MoUs between Mizoram University and farming societies (i.e. Tachhip Organic Growers Society and Tanhril Organic Growers Society) and providing them technology packages including biofertilizers to improve livelihood options for farming communities involved in shifting cultivation in Northeastern India. Any potential fungal strains identified in this study will be helpful in developing more suitable biofertilizer for better option to tribal farming communities to boost their farm income.

This project is looking at exploring the soil fungal abundance and diversity from bulk and rhizosphere samples at Wits Rural Facility a Lowveld Savannah Ecosystem in Limpopo, South Africa. The ecosystem is economically important to the region for animal and plant biodiversity, supporting ecotourism, farming, dry and wet wood harvesting. Sampling will be conducted following the SPUN sampling protocol on two forest patches of Terminalia sericea (silver cluster) and Dochrostyachys cinerea (sicklebush) and their adjacent open grass patches. The ecosystem is a biodiversity hotspot with the open grassy patches composed of Hyperthelia dissolute, Panicum maximum, Heteropogon contortus and Themeda triandra as the common grasses with few below ground biodiversity studies having carried out in the ecosystem. After DNA extraction, PCR amplification, sequencing will be done on both ITS2 and SSU using Illumina MiSeq platform, to explore the abundance and diversity of fungi with a special interest in arbuscular mycorrhiza in the ecosystem. Scientific impacts of the study will be to give further insight on the top-down effects of fire and encroachment on the ecosystem and its implications to below ground fungal diversity and a significant contribution to global diversity mapping. Social benefits will include the sharing of knowledge about fungi and soil sampling techniques to the local communities and how they can improve their management of the ecosystem for environmentally friendly future sustainability. 

The Amazon basin ranges from the Andes to the Atlantic Ocean. It is one of the most diverse biomes in the world and encompasses a series of ecosystems and vegetation types. The Amazon coastline is influenced by the Amazon River and its drainage basin, hosting about 85% of the mangroves and 35% of the sandy coastal plains (‘restingas’) of Brazil. Mangroves and restingas thrive under several limiting environmental factors and provide many ecosystem services, including recreation and tourism, soil protection, carbon sequestration, and nutrient cycling. They are also home to indigenous communities that have a deep connection with them. As these coastal ecosystems develop under unique environmental conditions, they are critically fragile and sensitive to human disturbances. The aim of this project is to identify the molecular diversity of the fungal community and its potential functional role in mangroves and restingas from Northeast Amazon. We also aim to identify indicator species or functional groups associated with their specific soil conditions, to support conservation and rehabilitation projects. The analysis of fungal diversity will allow us to understand the aspects that contribute to mangrove and restinga maintenance, tolerance, and even their resilience in the face of emerging environmental changes.

Charcoal production in southern Mozambique’s mopane woodlands, dominated by Colophospermum mopane, has led to widespread ecosystem disturbance. These woodlands, which are ecologically and economically significant, are increasingly degraded due to overharvesting and expanding urban charcoal demand. This study explores how such disturbances affect the diversity and composition of mycorrhizal fungal communities, which are vital for soil health and vegetation regrowth. Using a chronosequence approach in the Mabalane District (23.8478° S, 32.6249° E), we will assess fungal communities across abandoned charcoal kiln sites of varying ages and compare them to pristine mopane areas. Soil samples will be collected from 80 plots and analyzed using ITS region metabarcoding, with fungal identification performed via the UNITE database. Vegetation surveys will complement fungal data to examine regeneration patterns. Our research aims to determine whether passive restoration can support fungal and ecosystem recovery. Outcomes will inform sustainable land-use practices, conservation efforts near key biodiversity areas such as Kruger National Park, and policies aligned with REDD+ objectives. By integrating ecological, molecular, and spatial data, this work supports strategies for balancing charcoal production with the long-term resilience of mopane ecosystems—benefiting local livelihoods, biodiversity conservation, and broader environmental sustainability in semi-arid southern Africa.

Our project will contribute to map several under-sampled areas, ecosystems and habitats in Bulgaria, which are also major biodiversity hotspots and conservation sites within Europe. We will provide essential mycorrhizal biodiversity reference data points, while potentially identifying new mycorrhizal biodiversity hotspots. The local impact will be tremendous as mycorrhizal research in Bulgaria is underdeveloped. We will establish new research directions and build state-of-the-art expertise, especially in molecular ecology.

Northeast India is situated at the confluence of Indo-Malayan, Indo-Chinese and Indian bio- geographical realms and as a result of this the region harbours rich and diverse culture and high biodiversity and endemism. The region’s vegetation types range from tropical rain forest in the foothills to Alpine meadows and cold deserts which amount to more than one-third of the country's total biodiversity. The region is an important part of the Indo-Myanmar biodiversity hotspot, one of the 12 mega biodiversity hotspots of the world and represents 50 percent of Indian biodiversity. Seven hill states that include Arunachal Pradesh, Manipur, Meghalaya, Mizoram, Nagaland, Sikkim and Tripura in the region are collectively referred to as North Eastern Hill Region (NEHR). It is located between of 21°58' to 29°30'N latitude and 88°58' to 97°30' E longitude and is spread over 1, 83,741 km2 area. Climate of the region varies from tropical to alpine type with a very high range of variation in precipitation. AM fungi are obligate symbionts, their population and diversity may be determined by the plant species present in the given ecosystem. The hilly terrain of NEH interspersed with valleys and plains; the altitude varies from almost sea-level to over 7,000 meters above MSL. The change in elevation and climate allowed vegetation/forests of six major categories viz., tropical, subtropical, temperate, montane, subalpine and alpine. Above ground floristic diversity impacts below ground microbiome especially AMF. Information on the distribution and frequency of occurrence of specific AM fungi in the Indo Myanmar Biosphere of the NEH area is very scarce. Hence, we hypothesize to study the abundance and diversity of AMF across gradients in the Indo Myanmar Biosphere of the NEH in the light of changes in elevation and climatic variations in interactive and intra-active mode with native macroflora and microflora respectively.

Land use types influences the diversity of mycorrhizal fungi. Our project aims at sampling the mycorrhizal diversity in three land use types (natural forest reserve, tree plantation, and grazing grassland) in the Highlands Forests of Cameroon. The area consists of mixed forest and savannah grasslands with some of the highest levels of endemism in the Western Highlands. This area is a biodiversity hotspot of global significance that supports a high diversity of animal and plant species, large numbers with restricted ranges, and many of which are threatened and critically endangered. The natural habitats are encroached on and are shrinking. Research on fungi diversity in this area is limited with few studies. With our team, including landscape ecologists, mycology expert, postdoctoral researcher, PhD and MSc students as well as local community members, we will carryout a field sampling according to SPUN standardized protocol. A total of 30 soil samples and 20 ectomycorrhizal fruit bodies will be collected from three land use types. The ITS region of the DNA and high-throughput sequencing technique of the Illumina MiSeq metabarcoding on nuclear ribosomal ITS2 region will be done. Field sampling and results will be discussed with local communities and conservation groups to reinforce soil conservation in the Bamenda highlands, and the communities will learn more about the importance of mycorrhizal in general. Therefore, the scientific data will be shared with the communities in the Bamenda highlands to speak more loudly about the importance of mycorrhizal networks. This project will add crucial data to the map of fungal diversity in Cameroon, targeting the different land use types where the underground fungi have so far never been explored. The data obtained from this study will not only provide insight on how different land use influences mycorrhizal diversity but also contribute to the conservation and management of these ecosystems, ultimately fostering ecological resilience. The results will provide useful information on key fungal components to be further exploited as indicators in a sustainable ecosystem management perspective.

Can Gio mangrove forest covers a natural area of 70,445.20 hectares. In light of current climate change, the role and benefits of mangrove forests are becoming increasingly valuable and practical. Arbuscular mycorrhizal fungi (AMF) represent a symbiotic association between plants and root fungi that is very common in nature. This demonstrates the intimate interaction between AMF and the microbial community, with the composition of AMF influencing both the function and the response of species to current climate change conditions. Thirty soil samples will be collected from Can Gio, and each soil sample will be used to isolate mycorrhizal spores. All collected spores will then be subjected to DNA extraction using the Qiagen DNA extraction kit, following the manufacturer’s protocol. The Small Subunit (SSU) gene region will be amplified for metabarcoding, and Illumina sequencing will be used as the sequencing platform. Mycorrhizal fungi will be identified from the SSU sequences using LotuS2. The coordinates of soil sampling points for AMF isolation will be recorded based on a grid system or the geographic characteristics of the Can Gio area. The results will be exported as a raster map illustrating the probability of AMF presence across the study area.

EAST DECCAN DRY EVERGREEN FOREST OF INDIA (EDDEFI) is Unique in its character as they are confined to a part of the Peninsular India (25458 sq.km spread over). EDDEFI is present along the coast of Bay of Bengal extending from Nellore of Andhra Pradesh and towards Tanjore District in Tamil Nadu state, India. The forest is restricted within the range of 30 to 100 km away from the coast with the stretch of more than 500km uniquely possessing evergreen plants. 1200mm - 1400mm rainfall, 75 - 85% humidity, 17oC in winter and 45oC in summer as temperature and dews from December to February are characteristics of EDDEFI. Small part of these sort of vegetation is found to strive at these environment making EDDEFI as Unique in its ecosystem at global level. The forest is highly threatened by local communities where only 5 % of the EDDEFI is possessing the natural vegetation. Currently we are exploring the beautiful Eco-region for the presence of Mycorrhiza.

The Selva Paranaense is home to the entire Atlantic forest of Argentina, one of the largest remaining virgin forests in the world. The Selva Paranaense is home to 52% of Argentina’s biodiversity, with more than 150 species of mammals, 564 species of birds, 260 species of freshwater fish, 116 species of reptiles, 68 species of amphibians, and thousands of species of plants and fungi. Due to the high levels of biological diversity and its many endemic species, it is classified as a global biodiversity hotspot. The Paranaense Forest faces serious threats of deforestation from agricultural expansion and the invasion of introduced and invasive species. In the last 120 years, 95% of the native forest has been lost, severely affecting the region’s flora and fauna. It should be noted that a large number of plant species have not yet been fully classified. The counterpart of the enormous plant diversity remains undiscovered: the arbuscular mycorrhizal fungi. These essential actors of nature have not been studied so far in crucial national and provincial reserves. Thanks to SPUN, we will unveil the underground networks of mycorrhizal fungi in these priceless relics.

Sulawesi is an island located between two oceanic trenches that separate the Asian and Australian continental shelves. Due to its geographic isolation and extraordinary geology, Sulawesi has developed very distinctive flora and fauna, becoming a hotspot of endemic species (Whitten et al., 2002, Middleton et al 2019, Butarbutar et al., 2022). Two distinct ecoregions can be found in Sulawesi: lowland rain forest (LRF) and montane rain forest. For our sampling campaign we have identified a location for its unique concentration of endemic and threatened species: Rawa Aopa Watumohai National Park (in LRF, South East Sulawesi). The park presents mangrove forests, coastal forests, savanna freshwater swamp forests, which are our main focus for this research. We will collect soil samples, vegetation and mushroom specimens. DNA will be extracted from soil samples, and ITS, SSU and 16S rRNA regions will be amplified and sequenced with Oxford Nanopore Technology (ONT) MinION. We expect that the remarkable diversity found in flora and fauna is reflected in the underground microbial community of Sulawesi swamp forests and that undiscovered taxa can be found in the pristine forest sites. Students and researchers from Tadulako University, IPB University and Indonesian Native Plant Foundation will collaborate on this project.

Plant-fungal interactions, such as mycorrhizal symbiosis, are major determinants of plant biogeographic range size, population dynamics and plant community composition. In tropical forest ecosystems of oceanic islands, native and endemic plant communities tend to be replaced by introduced and invasive plant communities resulting in significant changes in the overstory, understory and forest floor. However, in these regions of the world, the impact of biological invasions on soil fungal biodiversity is poorly documented. In this project, we propose to (1) conduct a molecular inventory of soil fungi in the rainforests of Mo'orea, one of the high islands of French Polynesia and (2) assess the impact of the invasive plants Miconia calvescens and Spathodea campanulata on these fungal communities.

The taxonomic inventory of fungal species will be based on high-throughput sequencing techniques of ribosomal DNA (metabarcoding). It will be conducted on DNA extracted from the soils of the high altitude rainforest called "cloud forest" and in the low altitude mesophilic forest on an experimental site listed as a natural area of ecological and heritage interest (ENIEP of Opunohu). The aim is to evaluate the impact of biotic factors (e.g. presence of invasive plants) and abiotic factors (e.g. level of precipitation) on the composition and richness of soil fungi communities (saprotrophs and mycorrhizal symbiotics).A better knowledge of fungal communities in forest soils and endemic tree roots should inform us on the effects of fungal microbial communities on the vulnerability and resilience of tropical rainforests to plant invasions, in a context of climate change.

Cliffs are extreme environments that hold unique and diverse plant communities with a great ability to adapt to their particular severe conditions. Cliffs hold specialist plant species, many of them being endemic and threatened plant species. Mutualistic interactions with mycorrhizal fungi may favor their adaptation, as they can ameliorate plants toward biotic stress mitigation or efficient water nutrient acquisition. Moreover, considering the potential specific selectivity of arbuscular mycorrhizal fungi (AMF) to cliff plant hosts, the cliff mycorrhizal community may be unique. However, these questions remain largely unexplored. An in-depth understanding of cliff microbiota and their distinctive and specific interactions with cliff plants is key to understanding this unexplored system. Considering all this, we hypothesize that: H1.-Cliff-specialist plants hold specific mycorrhizal communities that favour their adaptation to cliffs environments. H2.-Generalist species inhabiting cliffs form different mycorrhizal symbioses than the same species growing on a horizontal stratum in the vicinity of the cliff.

The Chota-Nagpur ecoregion, a part of the Deccan Peninsula Biogeographical Zone in the Indo-Malayan realm, spans 122,419 km² and comprises a tapestry of landscapes—from lateritic plateaus and rugged ravines to dramatic cliffs, deep alluvial plains, gorges, and monadnocks. The ecoregion, characterized by a tropical monsoon climate with distinct dry and wet seasons, is home to Asia's largest Sal (Shorea robusta) dry deciduous forests. Yet, despite its ecological richness, the fungal biodiversity of this ecoregion remains largely unexplored, presenting a unique opportunity to explore its diverse soil fungal communities. Mycorrhizal fungi, in particular, are invaluable bioindicators of forest health, but despite their ubiquity and importance, they often remain unseen, neglected, and not studied. Regrettably, forest fires, climate change, and extractive industry activities are causing fungi to become extinct at an accelerated rate. Therefore, a dedicated green restoration and conservation study is urgently needed. This soil mycobiome consortium study can effectively map and identify new species while providing insights into the hidden ecological dynamics, allowing us to harness them as a climate solution before they go extinct. Protecting these tiny, invisible underground fungi not only safeguards the forest land from extractive industries but also enhances the broader environmental context.

Tropical Andean montane forests are hotspots of endemic plant species and provide a number of ecosystem services in terms of biodiversity, water, and carbon sequestration. The Andean timberline ecosystems in upper montane forests have been inhabited since prehistoric times for grazing and agriculture activities for ancient civilizations. However, anthropic activities have increased to perpetrate illegal mining and new cropland frontiers, and the long-period drought events caused by climate change increases the flammability of treelines for fire events. This study challenges the understanding of the soil microbiota diversity across undisturbed and disturbed treeline montane forest and grassland ecosystems along the eastern flank of the SE Peruvian Andes. The main hypotheses of this research pave the way to gather this understanding that arbuscular mycorrhizal fungi diversity decreases from montane cloud forest to puna grassland ecosystems, which will be related to grazing, fire events, abiotic factors, and plant diversity, and to what extent these patterns are determined by soil nutrients availability. Our outcomes will be engaged with national and international mycologists to expand and find new frontiers of knowledge about belowground soil biota for tropical regions, and this project is open to collaborate with other projects to understand the forest ecosystem functioning.

This project investigates the impact of traditional indigenous agroforestry on mycorrhizal fungal diversity in cacao cultivation within the Osa Peninsula, Costa Rica. Indigenous Térraba and Boruca communities manage cacao using ancestral techniques, which may enhance soil biodiversity compared to modern cultivation systems. Through metabarcoding of the 18S rRNA gene (SSU region) using Wanda primers and high-throughput sequencing (Illumina MiSeq), this study will assess fungal community composition across 50 sites. Bioinformatics analysis will leverage Öpik and Maarjam databases for species identification. Complementary morphological and molecular analyses will provide a comprehensive characterization of mycorrhizal diversity. Findings will offer insights into sustainable soil management, contributing to conservation efforts and aligning with UN Sustainable Development Goals on resilient agriculture and land preservation. The research will also empower indigenous farmers with knowledge on soil microbiome dynamics, strengthening agroforestry systems and local economies. Expected outcomes include a peer-reviewed scientific publication, workshops for indigenous communities, and practical recommendations for integrating traditional practices into modern cacao cultivation. By bridging ancestral knowledge with scientific research, this project aims to promote biodiversity conservation and sustainable agricultural practices in tropical ecosystems

The idea of our project within SPUN is to provide evidence of root mycobiome biodiversity around monumental trees forming the backbone of East Carpathian Biosphere Reserve. Polish NGOs have provided us with GPS coordinates of 3675 ‘monumental trees’ which should be considered as the ‘hot spots’ of the region, but still are being logged there at an alarming rate.

We intend to support the initiative to include this priceless sites in the Bieszczady National Park. Therefore, we want to share the scientific data with the activist and artistic communities in Poland to speak more loudly about the importance of forest mycorrhizal networks.