Underground Explorers

Despite the important ecological functions played by arbuscular mycorrhizal fungi (AMF), few studies have been conducted on AMF associated with plant species particularly baobab in Malawi. Furthermore, the ecology under-which baobab thrives support limited number of tree species. This proposed study therefore seeks to explore the possible role of AMF on growth and establishment of baobab species within the selected ecological sites. The study will be conducted in Malawi in the following districts: Mangochi, Karonga and Salima. The study will comprise field soil sampling that will be done in collaboration with local communities followed by laboratory work. Field soil sampling will follow SPUNs standardized protocols and it is anticipated that atleast 30 soil samples will be collected. Laboratory work will involve DNA extraction, quantification, amplification as well as visualization of PCR products will locally be visualized using gel electrophoresis. Additionally, DNA will be sent to external laboratory for deep sequencing using oxford nanopore sequencing platform. The study will elucidate the genetic diversity of AMF associated with baobab and hence contribute towards biodiversity conservation strategies. Furthermore, the study will create awareness on the roles of AMF on baobab species and contributes towards the global science on AMF through publications and video clips.